Abstract
Recently, a method to encode unnatural amino acids with diverse physicochemical and biological properties genetically in bacteria, yeast and mammalian cells was developed. Over 30 unnatural amino acids have been co-translationally incorporated into proteins with high fidelity and efficiency using a unique codon and corresponding transfer-RNA:aminoacyl-tRNA-synthetase pair. This provides a powerful tool for exploring protein structure and function in vitro and in vivo, and for generating proteins with new or enhanced properties.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acids / chemistry*
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Amino Acids / genetics
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Amino Acyl-tRNA Synthetases / chemistry
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Amino Acyl-tRNA Synthetases / genetics
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Base Sequence
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Codon
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Escherichia coli / genetics
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Genetic Code*
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In Vitro Techniques
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Mutation
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Protein Biosynthesis / genetics*
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Protein Engineering / methods*
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Proteins / chemistry
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RNA, Transfer / chemistry*
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RNA, Transfer / genetics
Substances
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Amino Acids
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Codon
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Proteins
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RNA, Transfer
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Amino Acyl-tRNA Synthetases